CN101281194B - Blood cell analyzer cleaning fluid - Google Patents
Blood cell analyzer cleaning fluid Download PDFInfo
- Publication number
- CN101281194B CN101281194B CN2008101068947A CN200810106894A CN101281194B CN 101281194 B CN101281194 B CN 101281194B CN 2008101068947 A CN2008101068947 A CN 2008101068947A CN 200810106894 A CN200810106894 A CN 200810106894A CN 101281194 B CN101281194 B CN 101281194B
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- China
- Prior art keywords
- cleaning fluid
- blood cell
- sodium
- cell analyzer
- sodium chloride
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
A blood cell analyzer cleaning fluid is characterized in that one litre of cleaning fluid is provided with 10.0-4.0g of sodium chloride, 0-6.0g of sodium sulfate, 16.0-23.0g of plant prolease, 0.4-1.2g of complex enzyme stabilizer, 0.17-0.2g of sodium hypochlorite, 2.0-6.0g of anionic surfactant, a phosphate buffer toning the ph value to 8.0-9.0, and the balance is water, wherein, the adding amounts of sodium chloride and sodium sulfate are complementary, the sum of the two adding amounts is no more than 10g. The invention does not contain cyanide, azide, and has non-toxicity, which can effective improve working atmosphere of operating staff, and can reduce harm of toxicant to personal health; the physical chemistry characters of the reagent are stable, and the reagent can be stored in the room temperature for more than one year. The invention can not only secure testing result accuracy, but also can secure non-corrosiveness to the blood cell analyzer; and hs no pollution to the environment.
Description
Technical field
The invention belongs to the cleaning reagent of medical diagnostic equipment, particularly blood cell analysis is only used cleaning reagent.
Background technology
Cleaning fluid is one of reagent the most frequently used in the blood analyser, and its effect is blood stains and the fibrin in the cleanser conduit, guarantees the accuracy of testing result.With the blood cell analysis is example: blood samples in red blood cell and the white blood cell count(WBC) pond by suction needle after diluted, instrument will add hemolytic agent automatically, automatically count through instrument behind the certain hour, counting finishes, and is drawn pipeline and counting chamber and each passage of a certain amount of cleaning fluid flushing blood process automatically by the sample suction pump in the instrument.Repeat above working link, order ground is the accuracy of guaranteeing the quality inspection result.The past common method is: with concentrated cleaning solutions blood-counter system is concentrated immersion when the plug-hole warning occurring, though this method of operating can be dealt with problems, but the method effort is time-consuming again, and to environment, and operating personnel pollution is arranged, blood analyser is had bigger corrosivity.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, a kind of cleaning fluid that the cellanalyzer transducer potector cleans that is used for efficiently is provided.
Each material and component are as follows in the cleaning fluid of the present invention, in 1 liter of cleaning fluid:
Sodium chloride 10.0～4.0g
Sodium sulphate 0～6.0g
Plant rennet 16.0～23.0g
Complex enzyme stabilizing agent 0.4～1.2 g
Sodium hypochlorite 0.17～0.2g
Anionic surfactant 2.0～6.0g
Phosphate buffer is transferred PH8.0～9.0
Yu Weishui
Wherein the addition of sodium chloride and sodium sulphate is complementary, but both while addition sum≤10g.
Sodium hypochlorite of the present invention can be replaced with antiseptic, and its addition is 0.2～0.6g.
Sodium hypochlorite of the present invention and antiseptic also can add simultaneously by sodium hypochlorite 10.0～4.0g, antiseptic 0.2～0.6g, but both while addition sum≤0.8g.
Cleaning fluid of the present invention both had been applicable to cellanalyzer usefulness, also can be used for other testing instruments, as Biochemical Analyzer.
Plant rennet of the present invention can be various plant rennets such as subtilisin, soybean protein enzyme, shell-shaped proteinase.
Complex enzyme stabilizing agent of the present invention can be complex enzyme stabilizing agent DPD, complex enzyme stabilizing agent ADG, complex enzyme stabilizing agent AES etc.
Antiseptic of the present invention can be to mix phenol, 3,5 two chloro-2-DHBSs, parachlorphenol etc.
The present invention does a lot of work selecting for use on enzyme stabilizers and the plant rennet, utilize triton as screening agent parcel enzyme molecule, the environment of phosphate-buffered to providing suitable enzyme to preserve, sodium chloride and sodium sulphate provide required electricity to lead for cleaning instrument, enzyme stabilizers guarantees that further enzyme is not influenced by surrounding enviroment, and sodium hypochlorite and antiseptic then are the bacterial growths that suppresses in the peripheral liquid.
Use cleaning fluid of the present invention, can must clean 1～3 time with concentrating by testing 50 whole blood samples existing every day, be reduced to and test 50 whole blood samples every day every concentrated the cleaning 1 time of a week usefulness, even can clean once with concentrating every two weeks, alleviate operator's labour intensity effectively, prolonged the life-span of instrument.
The present invention does not contain trinitride, and avirulence can effectively be improved operating personnel and producers' working environment, reduces the injury of poisonous substance to person health, and its physicochemical property are stable, and reagent at room temperature can be stored more than 1 year.Can guarantee the accuracy of testing result, can guarantee again the cellanalyzer non-corrosiveness, and environmentally safe.
Embodiment
The present invention will be described further by following examples.
Embodiment 1.
In pure water, add sodium chloride 10g, shell-shaped proteinase 16g, complex enzyme stabilizing agent AES0.4g, sodium hypochlorite 0.2g, activating agent 4g, transfer PH8.7 with phosphate buffer, simultaneously with pure water quantitatively to one liter, filter.
Embodiment 2.
In pure water, add sodium chloride 7g, sodium sulphate 3g, soybean protein enzyme 20g, complex enzyme stabilizing agent ADG0.8g, sodium hypochlorite 0.15g, anionic surfactant 4g, antiseptic mixing phenol 0.4g, transfer PH8.0 with phosphate buffer, simultaneously with pure water quantitatively to one liter, filter.
Embodiment 3.
In pure water, add sodium chloride 4g, sodium sulphate 6g, subtilisin 23g, complex enzyme stabilizing agent DPD1.2g, sodium hypochlorite 0.17g, anionic surfactant 6g, antiseptic 3,5 two chloro-2-DHBS 0.2g, transfer PH9.0 with phosphate buffer, simultaneously with pure water quantitatively to one liter, filter.
Claims (2)
1. blood cell analyzer cleaning fluid is characterized in that in 1 liter of cleaning fluid:
Sodium chloride 10.0～4.0g
Sodium sulphate 0～6.0g
Plant rennet 16.0～23.0g
Complex enzyme stabilizing agent 0.4～1.2g
Sodium hypochlorite 0.17～0.2g
Anionic surfactant 2.0～6.0g
Phosphate buffer is transferred PH8.0～9.0
Yu Weishui
Wherein the addition of sodium chloride and sodium sulphate is complementary, and both are addition sum≤10g simultaneously.
2. cleaning fluid according to claim 1 is characterized in that substituting sodium hypochlorite with antiseptic, and the addition of antiseptic is 0.2～0.6g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101068947A CN101281194B (en) | 2008-06-04 | 2008-06-04 | Blood cell analyzer cleaning fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101068947A CN101281194B (en) | 2008-06-04 | 2008-06-04 | Blood cell analyzer cleaning fluid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101281194A CN101281194A (en) | 2008-10-08 |
| CN101281194B true CN101281194B (en) | 2010-11-10 |
Family
ID=40013758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101068947A Expired - Fee Related CN101281194B (en) | 2008-06-04 | 2008-06-04 | Blood cell analyzer cleaning fluid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101281194B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104059794A (en) * | 2014-06-25 | 2014-09-24 | 南通永康检测技术有限公司 | Cleaning fluid of analyzer |
| CN104017665A (en) * | 2014-06-25 | 2014-09-03 | 南通永康检测技术有限公司 | Cleaning fluid |
| CN106635440A (en) * | 2016-12-19 | 2017-05-10 | 青岛古高生物科技有限公司 | Cleaning liquid of blood coagulation analysis instrument |
| CN107402280B (en) * | 2017-06-06 | 2019-05-31 | 金华市鑫科医药科技有限公司 | A kind of cellanalyzer cleaning solution cleaning rate measuring method |
| CN108562754A (en) * | 2018-01-17 | 2018-09-21 | 河北睿达模生物科技有限公司 | Hepatitis B e antigen individual event quality-control product and its preparation process |
| CN109142196A (en) * | 2018-08-18 | 2019-01-04 | 山东博科生物产业有限公司 | Three classification reagent for hemocyte analyzers of one kind |
| CN113004983A (en) * | 2019-12-20 | 2021-06-22 | 深圳市帝迈生物技术有限公司 | Cleaning solution, method for producing cleaning solution, and blood cell analyzer |
| CN114686318A (en) * | 2022-03-28 | 2022-07-01 | 内江市第二人民医院 | Hemocyte analyzer cleaning solution and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4745071A (en) * | 1985-09-05 | 1988-05-17 | Sequoia-Turner Corporation | Method for the volumetric differentiation of blood cells types |
| CN1037776A (en) * | 1988-05-18 | 1989-12-06 | 中国人民解放军第二三○医院 | PC-703 type electronic blood cell counter reagent process |
| CN1222677A (en) * | 1997-10-31 | 1999-07-14 | 中国人民武装警察部队江苏省总队医院 | Diluent and blood solvent cleaning liquid for blood cell counter and their preparation |
| CN1238455A (en) * | 1998-06-04 | 1999-12-15 | 梁建华 | Diluent and cleaning liquid for blood analysis counter |
| US6165966A (en) * | 1996-09-24 | 2000-12-26 | The Procter & Gamble Company | Liquid detergents containing proteolytic enzyme and protease inhibitors |
| CN1424582A (en) * | 2002-12-26 | 2003-06-18 | 王传新 | Yapei 3700 automatic unsorted blood cells analystic instrument reagent |
| US20070238632A1 (en) * | 2006-04-07 | 2007-10-11 | Beckman Coulter, Inc. | Formaldehyde-free cleaner composition for cleaning blood analyzers and method of use |
-
2008
- 2008-06-04 CN CN2008101068947A patent/CN101281194B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4745071A (en) * | 1985-09-05 | 1988-05-17 | Sequoia-Turner Corporation | Method for the volumetric differentiation of blood cells types |
| CN1037776A (en) * | 1988-05-18 | 1989-12-06 | 中国人民解放军第二三○医院 | PC-703 type electronic blood cell counter reagent process |
| US6165966A (en) * | 1996-09-24 | 2000-12-26 | The Procter & Gamble Company | Liquid detergents containing proteolytic enzyme and protease inhibitors |
| CN1222677A (en) * | 1997-10-31 | 1999-07-14 | 中国人民武装警察部队江苏省总队医院 | Diluent and blood solvent cleaning liquid for blood cell counter and their preparation |
| CN1238455A (en) * | 1998-06-04 | 1999-12-15 | 梁建华 | Diluent and cleaning liquid for blood analysis counter |
| CN1424582A (en) * | 2002-12-26 | 2003-06-18 | 王传新 | Yapei 3700 automatic unsorted blood cells analystic instrument reagent |
| US20070238632A1 (en) * | 2006-04-07 | 2007-10-11 | Beckman Coulter, Inc. | Formaldehyde-free cleaner composition for cleaning blood analyzers and method of use |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101281194A (en) | 2008-10-08 |
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Effective date of registration: 20091211 Address after: Dongsheng Road 699, Chang Dong Industrial Zone, Jiangxi, Nanchang: 330012 Applicant after: Nanchang Biotech A & C Biotechnical Industry Incorporated Company Address before: Jiangxi province Nanchang Liberation Road No. two Middle East Chang Baxter Industrial Park Post encoding: 330012 Applicant before: Nanchang Bt Biology High & New Technology Co., Ltd. |
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Granted publication date: 20101110 Termination date: 20200604 |
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