CN101281193B - Blood cell analyzer dilution - Google Patents
Blood cell analyzer dilution Download PDFInfo
- Publication number
- CN101281193B CN101281193B CN2008101068932A CN200810106893A CN101281193B CN 101281193 B CN101281193 B CN 101281193B CN 2008101068932 A CN2008101068932 A CN 2008101068932A CN 200810106893 A CN200810106893 A CN 200810106893A CN 101281193 B CN101281193 B CN 101281193B
- Authority
- CN
- China
- Prior art keywords
- sodium
- dilution
- hemolytic agent
- addition
- blood cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
A blood cell analyzer diluent and a hemolytic agent, are characterized in that one litre diluent is provided with 12.0-4.0g of sodium chloride, 2.0-10.0g of sodium sulfate, 0.8-0.5g of 1,3,2-methylol urea, 0.2-0.5g of copper sulfite, 3.0-8.0g of EDTA-2Na, 0.2-0.7g of Piperacillin Sodium, a borate buffering liquid toning the ph value to 7.2-7.8, and the balance is water; one litre hemolytic agent is provided with 0.8-5.0g of potassium chloride, 0-60.0g of dodecyl trimethyl ammonium chloride, 14.0-0g of octadecyl trimethyl ammonium bromide, 6.0-10.0ml of isopropanol, carbonate or alcaine buffering liquid toning the ph value to 7.2-7.8, and the balance is water. The inventive reagent can form stable hemoglobin derivatives, and the absorption spectrum curves are similar when lambada is 540nm,lambada is 504nm, which can satisfy the clinical inspection requirement; the reagent does not contain cyanide, azide, and has non-toxicity, which can effective improve working atmosphere of operatingstaff, and can reduce harm of toxicant to personal health.
Description
Technical field
The invention belongs to medical diagnostic equipment reagent field, particularly reagent for hemocyte analyzers.
Background technology
Dilution and hemolytic agent are the most frequently used group reagents of blood analyser in the medical test, are the key reagents of measuring haemoglobin (Hb) and leucocyte (WBC) counting or classification.Hemolytic agent is under the mating reaction of dilution, make red blood cell (RBC) dissolving rapidly on the one hand, discharge haemoglobin and combine the stable haemoglobin dervative of formation with hemolytic agent, for quantitative measurement Hb, leucocyte is kept original form on the other hand, and keep certain volume, for WBC counting usefulness, and come leucocyte is carried out three hive off (lymphocyte, neutrophil leucocyte, osculant cells) according to leukocytic size.1996, ICSH recommended cyanmethemoglobin (HiCN) determination method as international Hb bioassay standard method, and its Main physical index is: at crest λ=540nm, during trough λ=504nm, have tangible crest, trough.Though this method can more accurately be measured Hb; But can not measure WBC simultaneously, thereby, can't be applied to clinical.
At present, it is to add the potpourri that potassium cyanide (KCN) is formed with organic quaternary amine that hemolytic agent commonly used is gone up in clinical medical inspection both at home and abroad, its absorption spectrum and Hi CN are comparatively approaching on the one hand, on the other hand, after organic quaternary amine destroys RBC, Hb and KCN generate stable CNHb rapidly, reach the purpose of measuring Hb, do not destroy WBC simultaneously again.But the disadvantage of the hemolytic agent of this compatibility is KCN severe toxicity is arranged, not only serious harm laboratory worker healthy, to also bringing a lot of inconvenience in the production unit operation, environmental pollution is also very serious simultaneously.Pollute measure though formulated very strict, loaded down with trivial details reduction cyanogen clinically, its pollution to the person, environment still can't be avoided.
Disclose a kind of cyanogens-free hemolytic agent among the Chinese patent CN99115572.6, but it can only use in CD series, F series blood cell analysis instrument, can not use in other serial instrument.And the dilution that does not match (Dil).Water used in the preparation must be double distilled water, operating difficulties in actual production, cost height.And the cetyl trimethyl ammonium bromide light specific gravity, there is suspended powder floating in air during preparation.This product sucks the human body harmful, be generalized to have some problems in the practical application, and its range of application also is restricted.Fundamentally do not solve the blood test personnel and comprise that producers' health is subjected to the difficult problem of toxin infringement, such as pollution, the cyanide pollution of trinitride, the cetyl powder is to operator's pollution in the preparation.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art and a kind of RBC that can destroy rapidly is provided, do not damage WBC again, and its absorption spectrum is near HiCN, azide-free thing dilution and cyanogens-free hemolytic agent that physicochemical property are stable nontoxicly are convenient to operation again.
Each material and component are as follows in the dilution of the present invention,
In 1 liter of dilution:
Sodium chloride 12.0～4.0g
Sodium sulphate 2.0～10.0g
One or three dimethyl ureas, 0.8～0.5g
Sulphurous acid copper 0.2～0.5g
Disodium ethylene diamine tetraacetate 3.0～8.0g
XiLin sodium 0.2～0.7g draws in group
It is 7.2～7.8 that borate buffer solution is transferred PH
Yu Weishui
Wherein the addition of sodium chloride and sodium sulphate is complementary, but both addition sum≤14g; The addition of one or three dimethyl ureas and sulphurous acid copper is complementary, but both addition sum≤1.0g.
XiLin sodium draws in group in the dilution of the present invention, can substitute with sodium fluoride, and its addition is 0.3～0.8g.
Group of the present invention draws XiLin sodium and sodium fluoride also can add simultaneously, but both addition sum≤1.0g.
Each material and component are as follows in the hemolytic agent that uses with dilution of the present invention pairing,
In 1 liter of hemolytic agent:
Potassium chloride 0.8～5.0g
DTAC 0～60.0g
Octadecyl trimethylammonium bromide 14.0～0g
Isopropyl alcohol 6.0～10.0ml
Carbonate or hydrochloride buffer are transferred PH3.2～5.8
Yu Weishui
Wherein the addition of DTAC, octadecyl trimethylammonium bromide is complementary, but both addition sum≤60g.
Carbonate of the present invention or hydrochloride buffer are transferred PH3.2～5.8 of hemolytic agent, are meant when the aforementioned all kinds of materials that add to make hemolytic agent greater than 5.8 the time, with hydrochloride buffer accent PH to 3.2～5.8; When the aforementioned all kinds of materials that add make hemolytic agent less than 3.2 the time, transfer PH to 3.2～5.8 with carbonate buffer solution.
Dilution of the present invention and hemolytic agent are applicable to HL-series cellanalyzer, also can be used for other cellanalyzer.
The present invention utilizes the cuprammonium salt replacement potassium cyanide of potassium chloride and metal ion masking agent owing to adopt such scheme, make itself and reaction of blood after, form stable haemoglobin dervative, and at λ=540nm, during λ=504nm, absorption spectrum curve is similar, can satisfy the needs of clinical examination; Simultaneously, do not contain prussiate in the reagent, do not contain trinitride, avirulence can effectively be improved operating personnel's working environment, reduces the injury of poisonous substance to health of human body.
Embodiment
Embodiment 1.
(1) preparation of dilution: in pure water, add sodium chloride 6.0g respectively, sodium sulphate 5.8g, one or three dimethyl urea 0.8g, sulphurous acid copper 0.2g, disodium ethylene diamine tetraacetate 3g, XiLin sodium 0.2g draws in group, dissolving, adding sodium borate buffer liquid accent PH is 7.3, quantitative one liter with pure water, filters.
(2) preparation of hemolytic agent: in pure water, add potassium chloride 3.0g respectively, DTAC 50g, octadecyl trimethylammonium bromide 8g, isopropyl alcohol 10ml, dissolving transfers PH 5.3 with carbonate 0.34g,, filters quantitatively to one liter with pure water.
Embodiment 2.
(1) preparation of dilution: in pure water, add sodium chloride 4.0g respectively, sodium sulphate 10g, one or three dimethyl urea 0.5g, sulphurous acid copper 0.5g, disodium ethylene diamine tetraacetate 3g, XiLin sodium 0.25g draws in group, dissolving, adding sodium borate buffer liquid, to transfer PH be 7.5, with pure water quantitative one liter simultaneously, filters.
(2) preparation of hemolytic agent: in pure water, add potassium chloride 0.9g respectively, DTAC 60g, isopropyl alcohol 6ml, dissolving transfers PH 4.5 with sodium bicarbonate, simultaneously with pure water quantitatively to one liter, filter.
Embodiment 3.
(1) preparation of dilution: in pure water, add sodium chloride 5.0g respectively, sodium sulphate 9g, one or three dimethyl urea 0.6g, sulphurous acid copper 0.3g, disodium ethylene diamine tetraacetate 5g, XiLin sodium 0.5g draws in group, dissolving, adding sodium borate buffer liquid accent PH is 7.8, quantitative one liter with pure water, filters.
(2) preparation of hemolytic agent: in pure water, add potassium chloride 2g respectively, DTAC 40g, octadecyl trimethylammonium bromide 4g, isopropyl alcohol 9ml, dissolving, transferring PH with carbonate is 4.0, simultaneously with pure water quantitatively to one liter, filter.
Embodiment 4.
(1) preparation of dilution: in pure water, add sodium chloride 8g respectively, sodium sulphate 5g, one or three dimethyl urea 0.5g, sulphurous acid copper 0.5g, sodium fluoride 0.8g, disodium ethylene diamine tetraacetate 6g, dissolving, adding sodium borate buffer liquid, to transfer PH be 7.7, with pure water quantitative one liter simultaneously, filters.
(2) preparation of hemolytic agent: in pure water, add potassium chloride 5g respectively, octadecyl trimethylammonium bromide 14g, isopropyl alcohol 10ml, dissolving, transferring PH with carbonate is 5.2, simultaneously with pure water quantitatively to one liter, filter.
Embodiment 5.
Present embodiment is Application Example of the present invention and and contains the trinitride dilution and contain the contrast of prussiate hemolytic agent.Used dilution, the hemolytic agent of present embodiment is dilution, the hemolytic agent of pressing the proportioning preparation of embodiment 1.Contrast groups is conventional containing the trinitride dilution and contain the prussiate hemolytic agent.
The used instrument of present embodiment: HL-series cellanalyzer, 722 type spectrophotometers; Get 20 fens normal person's anti-freezing blood samples, two group reagents detect respectively 20 fens sample gained (leucocyte. red blood cell. haemoglobin. blood tip plate. lymphocyte. granulocyte) result carries out statistical procedures, looks into the P value greater than 0.5 with the t value, both do not have significant difference at conclusion.
Subordinate list 1 is for containing the trinitride dilution and containing the assay that the prussiate hemolytic agent is applied to blood routine examination.
Subordinate list 2 is applied to (do not contain the trinitride dilution and do not contain the prussiate hemolytic agent) assay of the blood routine examination of subordinate list 1 used blood specimen for dilution of the present invention, hemolytic agent.
Subordinate list 3 has prussiate and azide reagent and the present invention's (cyanide-free and nothing are selected nitride) reagent absorption peak to detect contrast table.Wherein A1 is for there being prussiate reagent, and A2 is a reagent of the present invention, and group is used mark this shop.
Table 1
Table 2
Table 3
Continuous table 3
Claims (3)
1. blood cell analyzer dilution is characterized in that in 1 liter of dilution:
Sodium chloride 12.0～4.0g
Sodium sulphate 2.0～10.0g
One or three dimethyl ureas, 0.8～0.5g
Sulphurous acid copper 0.2～0.5g
Disodium ethylene diamine tetraacetate 3.0～8.0g
XiLin sodium 0.2～0.7g draws in group
It is 7.2～7.8 that borate buffer solution is transferred PH
Yu Weishui
Wherein the addition of sodium chloride and sodium sulphate is complementary, both sum≤14g, and the addition of one or three dimethyl ureas and sulphurous acid copper is complementary, both sum≤1.0g.
2. dilution according to claim 1 is characterized in that and can also add sodium fluoride again by the amount of 0.8～0.3g, the addition complementation that XiLin sodium draws in its addition and group, both sum≤1.0g.
3. dilution according to claim 1 is characterized in that drawing XiLin sodium, addition with the alternative group of sodium fluoride is 0.3～0.8g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101068932A CN101281193B (en) | 2008-06-04 | 2008-06-04 | Blood cell analyzer dilution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101068932A CN101281193B (en) | 2008-06-04 | 2008-06-04 | Blood cell analyzer dilution |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101744218A Division CN101907621A (en) | 2008-06-04 | 2008-06-04 | Cyanogens-free hemolytic agent for hemocyte analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101281193A CN101281193A (en) | 2008-10-08 |
| CN101281193B true CN101281193B (en) | 2010-11-10 |
Family
ID=40013757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101068932A Expired - Fee Related CN101281193B (en) | 2008-06-04 | 2008-06-04 | Blood cell analyzer dilution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101281193B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103575579A (en) * | 2012-08-07 | 2014-02-12 | 上海睿康生物科技有限公司 | Diluent formula for bacterium channel of urine visible component analyzer |
| CN103004749A (en) * | 2012-12-17 | 2013-04-03 | 江苏美诚生物科技有限公司 | Diluent for hematology analyzer |
| CN104297134A (en) * | 2014-11-05 | 2015-01-21 | 深圳市开立科技有限公司 | Hemolytic agent and application thereof as well as classifying and counting method for white blood cells |
| CN106168554B (en) * | 2016-02-29 | 2019-10-18 | 迈克生物股份有限公司 | A kind of reagent for hemocyte analyzers |
| CN107015007B (en) * | 2017-03-29 | 2019-02-19 | 苏州康铭诚业医用科技有限公司 | A kind of hemoglobin concentration hemolytic agent |
| CN107976354A (en) * | 2017-11-22 | 2018-05-01 | 中山市创艺生化工程有限公司 | A kind of reagent for hemocyte analyzers |
| CN108872225A (en) * | 2018-07-17 | 2018-11-23 | 浙江亚培生物技术有限公司 | A kind of detection reagent and detection method detecting animal blood cell |
| CN109142196A (en) * | 2018-08-18 | 2019-01-04 | 山东博科生物产业有限公司 | Three classification reagent for hemocyte analyzers of one kind |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4745071A (en) * | 1985-09-05 | 1988-05-17 | Sequoia-Turner Corporation | Method for the volumetric differentiation of blood cells types |
| US4962038A (en) * | 1980-06-16 | 1990-10-09 | Coulter Electronics, Inc. | Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes |
| US5227304A (en) * | 1991-01-16 | 1993-07-13 | Sequoia Turner Corporation | Method for counting whole blood diluent and detergent reagent system |
| CN1238455A (en) * | 1998-06-04 | 1999-12-15 | 梁建华 | Diluent and cleaning liquid for blood analysis counter |
| CN1265196A (en) * | 1997-08-01 | 2000-08-30 | 库尔特国际公司 | Blood diluent |
| CN1424582A (en) * | 2002-12-26 | 2003-06-18 | 王传新 | Yapei 3700 automatic unsorted blood cells analystic instrument reagent |
| CN1548942A (en) * | 2003-05-09 | 2004-11-24 | 上海捷瑞医用试剂有限公司 | Diluent for blood cell analysis instrument |
| CN1834612A (en) * | 2006-04-29 | 2006-09-20 | 北京索通医疗技术有限公司 | Multifunction dilutent for blood cell analyzer and prepn. method |
| CN101021456A (en) * | 2007-02-12 | 2007-08-22 | 南昌大学 | Method for producing tripartite group blood cell analyser diluent |
-
2008
- 2008-06-04 CN CN2008101068932A patent/CN101281193B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4962038A (en) * | 1980-06-16 | 1990-10-09 | Coulter Electronics, Inc. | Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes |
| US4745071A (en) * | 1985-09-05 | 1988-05-17 | Sequoia-Turner Corporation | Method for the volumetric differentiation of blood cells types |
| US5227304A (en) * | 1991-01-16 | 1993-07-13 | Sequoia Turner Corporation | Method for counting whole blood diluent and detergent reagent system |
| CN1265196A (en) * | 1997-08-01 | 2000-08-30 | 库尔特国际公司 | Blood diluent |
| CN1238455A (en) * | 1998-06-04 | 1999-12-15 | 梁建华 | Diluent and cleaning liquid for blood analysis counter |
| CN1424582A (en) * | 2002-12-26 | 2003-06-18 | 王传新 | Yapei 3700 automatic unsorted blood cells analystic instrument reagent |
| CN1548942A (en) * | 2003-05-09 | 2004-11-24 | 上海捷瑞医用试剂有限公司 | Diluent for blood cell analysis instrument |
| CN1834612A (en) * | 2006-04-29 | 2006-09-20 | 北京索通医疗技术有限公司 | Multifunction dilutent for blood cell analyzer and prepn. method |
| CN101021456A (en) * | 2007-02-12 | 2007-08-22 | 南昌大学 | Method for producing tripartite group blood cell analyser diluent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101281193A (en) | 2008-10-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101281193B (en) | Blood cell analyzer dilution | |
| EP1298441B1 (en) | A cyanide-free lytic reagent composition and method for hemoglobin and cell analysis | |
| CN101231243B (en) | Agent and method for testing reticulocyte | |
| CN104698157B (en) | Agent for blood cell analyzer | |
| EP1877802B1 (en) | Cyanide-free lytic reagent composition and method of use for hemoglobin and white blood cell measurement | |
| DE69829606T2 (en) | Reagent and method for classification and enumeration of leukocytes | |
| EP0430719B1 (en) | Compounds and reagents for determination of reticulocytes | |
| JPH11507733A (en) | Reagents and methods for differential expression determination of leukocytes in blood | |
| CN1198144C (en) | Reagent for measurement of hemoglobin in blood sample and determination of white cells | |
| CN109655454A (en) | A kind of method of different valence state iron ion content in measurement feed | |
| JP4567082B2 (en) | Classification and counting method of erythroblasts | |
| CN101464453B (en) | A kind of hemolytic agent | |
| CN104789209B (en) | A kind of water solublity Cu based on rhodamine B2+, Hg2+probe and its preparation method and application | |
| Strik et al. | Automated cerebrospinal fluid cytology | |
| CN108872225A (en) | A kind of detection reagent and detection method detecting animal blood cell | |
| CN101907621A (en) | Cyanogens-free hemolytic agent for hemocyte analyzer | |
| Abdelhamid | General methods for detection and evaluation of nanotoxicity | |
| US6890756B2 (en) | Method of using cyanide-free lyse solution to emulate a cyanide-containing lyse solution in the measurement of hemoglobin | |
| Škrajnar et al. | A flow cytometric method for determination of the blood neutrophil fraction in rats | |
| CN103308703A (en) | Automatic analysis method of formaldehyde in water sample | |
| CN1139809C (en) | Non-toxic hemolytic agent for blood analysis | |
| CN1344933A (en) | Non-cyanide hemolytic agent for blood analysis | |
| CN1424582A (en) | Yapei 3700 automatic unsorted blood cells analystic instrument reagent | |
| WO2019222620A1 (en) | Green concentrated reagent for hematology systems | |
| CN109750029A (en) | A kind of non-jelly type urine DNA preservation solution suitable for genital tract detection of pathogens |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20091211 Address after: 699, Dongsheng Avenue, Chang Dong Industrial Zone, Jiangxi, Nanchang: 330012 Applicant after: Nanchang Biotech A & C Biotechnical Industry Incorporated Co. Address before: Jiangxi province Nanchang Liberation Road No. two Middle East Chang Baxter Industrial Park Post encoding: 330012 Applicant before: Nanchang Bt Biology High & New Technology Co.,Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101110 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |